Determination of leukocytes infiltrating central nervous system in experimental allergic encephalomyelitis (EAE) model

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Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Pamukkale Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Türkiye

Tezin Onay Tarihi: 2018

Tezin Dili: Türkçe

Öğrenci: Kemal Ertosun

Danışman: Alaattin Şen

Özet:

Most of the current knowledge and the research on multiple sclerosis is based on data obtained from the experimental allergic encephalomyelitis (EAE) model. The factors that play a crucial role in cell migration into the central nervous system are being investigated for development of and new treatments. Leukocyte infiltration into the CNS occurs in diseases such as multiple sclerosis (MS) and is responsible for degenerative inflammatory pathologies. Hence, the analysis of leukocyte populations infiltrated into the CNS is a frequent examination for understanding the pathogenic mechanisms of MS and for therapeutic purposes. In this study, a total of 50 C57BL6 mice at the age of 6-8 week were used to develop EAE by subcutaneous injection of the MOG35-55 peptide in complete Freud's adjuvant. Ten healthy mice of same age and strain were used as a control group. After induction of EAE (3.5 ± 0.5 clinical score) brain tissues were removed. Leucocytes were isolated applying density gradient centrifugation from brain tissues. CD45, CD3, Ly6G, Ly6C, CD11b expressions at cell surfaces were detected using specific antibodies at both control and EAE mice by flow cytometric analysis. The results have shown that there was no statistical difference among the animals in the CD45-bright Ly6G groups, but a significant increase in the CD11b+-Ly6C cells in EAE mice compared to healthy groups were observed. Our findings have shown that the leukocyte infiltration into the CNS plays a role in the pathogenesis of EAE and could be used to follow the progression of EAE.