Immunomagnetic separation of B type acute lymphoblastic leukemia cells from bone marrow with flow cytometry validation and microfluidic chip measurements

İÇÖZ K., EKEN A., Cinar S., Murat A., ÖZCAN S., ÜNAL E., ...More

SEPARATION SCIENCE AND TECHNOLOGY, vol.56, no.15, pp.2659-2666, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 56 Issue: 15
  • Publication Date: 2021
  • Doi Number: 10.1080/01496395.2020.1835983
  • Journal Name: SEPARATION SCIENCE AND TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, Analytical Abstracts, Applied Science & Technology Source, Biotechnology Research Abstracts, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Pollution Abstracts, DIALNET, Civil Engineering Abstracts
  • Page Numbers: pp.2659-2666
  • Keywords: B type acute lymphoblastic leukemia, immunomagnetic separation, flow cytometry, bone marrow, hematogone, microfluidic chip, MINIMAL RESIDUAL DISEASE, CONCURRENT DETECTION, ONCOLOGY-GROUP, MRD
  • Abdullah Gül University Affiliated: Yes

Abstract

In order to detect the blast cells from bone marrow of patients, one strategy is to first isolate the cells using immunomagnetic beads. The aim of this study was to report the experimental results of the immunomagnetic separation efficiency of the blast cells from bone marrow of pediatric leukemia patients. To test the efficiency of immunomagnetic separation, flow cytometry measurements at critical steps were performed. We here reported 94.5% capture efficiency for CD10 nano beads. Patients samples were also analyzed with a microfluidic chip to test the feasibility for further developments.