Nanomechanical biosensing with immunomagnetic separation

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Icoz K., Savran C.

APPLIED PHYSICS LETTERS, vol.97, no.12, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 97 Issue: 12
  • Publication Date: 2010
  • Doi Number: 10.1063/1.3489356
  • Journal Name: APPLIED PHYSICS LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: biological techniques, biomagnetism, biosensors, cantilevers, magnetic sensors, molecular biophysics, nanosensors, proteins, CANTILEVER, PROTEINS
  • Abdullah Gül University Affiliated: No

Abstract

We report a biosensing method that combines immunomagnetic separation and nanomechanical detection. In this method, same magnetic beads that are used to "fish" biomolecules from complex mixtures enable deflection of a cantilever structure upon excitation by an oscillating magnetic field. Biotin-coated magnetic beads were used to capture and separate streptavidin from serum. Streptavidin loaded magnetic beads were exposed to a differential cantilever system whose sensing arm was functionalized with biotin. The magnetic force applied on the streptavidin-beads resulted in differential cantilever deflections that could be detected down to 0.26 angstrom(rms) in air. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3489356]