Nanomechanical biosensing with immunomagnetic separation
APPLIED PHYSICS LETTERS, cilt.97, sa.12, 2010 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 97 Sayı: 12
- Basım Tarihi: 2010
- Doi Numarası: 10.1063/1.3489356
- Dergi Adı: APPLIED PHYSICS LETTERS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Anahtar Kelimeler: biological techniques, biomagnetism, biosensors, cantilevers, magnetic sensors, molecular biophysics, nanosensors, proteins, CANTILEVER, PROTEINS
- Abdullah Gül Üniversitesi Adresli: Hayır
Özet
We report a biosensing method that combines immunomagnetic separation and nanomechanical detection. In this method, same magnetic beads that are used to "fish" biomolecules from complex mixtures enable deflection of a cantilever structure upon excitation by an oscillating magnetic field. Biotin-coated magnetic beads were used to capture and separate streptavidin from serum. Streptavidin loaded magnetic beads were exposed to a differential cantilever system whose sensing arm was functionalized with biotin. The magnetic force applied on the streptavidin-beads resulted in differential cantilever deflections that could be detected down to 0.26 angstrom(rms) in air. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3489356]