A "moonlighting" dizinc aminopeptidase from Streptomyces griseus: Mechanisms for peptide hydrolysis and the 4x10(10)-fold acceleration of the alternative phosphodiester hydrolysis

Ercan A., Park H. I., Ming L.

BIOCHEMISTRY, vol.45, no.46, pp.13779-13793, 2006 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Review
  • Volume: 45 Issue: 46
  • Publication Date: 2006
  • Doi Number: 10.1021/bi061086x
  • Journal Name: BIOCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.13779-13793
  • Abdullah Gül University Affiliated: No


A unique "enzyme catalytic promiscuity" has recently been observed, wherein a phosphodiester and a phosphonate ester are hydrolyzed by a dinuclear aminopeptidase and its metal derivatives from Streptomyces griseus ( SgAP) [ Park, H. I., Ming, L.-J. ( 1999) Angew. Chem., Int. Ed. Engl. 38, 29142916 and Ercan, A., Park, H. I., Ming, L.- J. ( 2000) Chem. Commun. 2501- 2502]. Because tetrahedral phosphocenters often serve as transition- state inhibitors toward the hydrolysis of the peptide, phosphoester hydrolysis by peptidases is thus not expected to occur effectively and must take place through a unique mechanism. Owing to the very different structures and mechanistic requirements between phosphoesters and peptides during hydrolysis, the study of this effective phosphodiester hydrolysis by SgAP may provide further insight into the action of this enzyme that is otherwise not obtainable from regular peptide substrates. We present herein a detailed investigation of both peptide and phosphodiester hydrolyses catalyzed by SgAP. The latter exhibits a first-order rate enhancement of 4 x 10(10)-fold compared to the uncatalyzed reaction at pH 7.0 and 25 degrees C. The results suggest that peptide and phosphodiester hydrolyses by SgAP may share a common reaction mechanism to a certain extent. However, their differences in pH dependence, phosphate and fluoride inhibition patterns, and proton inventory reflect that they must follow different pathways. Mechanisms for the two hydrolyses are drawn on the basis of the results, which provide the foundation for further investigation of the catalytic promiscuity of this enzyme by means of physical and molecular biology methods. The catalytic versatility of SgAP suggests that this enzyme may serve as a unique "natural model system" for further investigation of dinuclear hydrolysis. A better understanding of enzyme catalytic promiscuity is also expected to shed light on the evolution and action of enzymes.