Preparation of antibacterial electrospun poly(D, L-lactide-co-glycolide)/gelatin blend membranes containing Hypericum capitatum var. capitatum

AKŞİT N. N., GÜRDAP S., DİNÇER İŞOĞLU S., İŞOĞLU İ. A.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, vol.70, no.11, pp.797-809, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 70 Issue: 11
  • Publication Date: 2021
  • Doi Number: 10.1080/00914037.2020.1765354
  • Journal Name: INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chimica, Compendex, INSPEC
  • Page Numbers: pp.797-809
  • Keywords: Electrospinning, Hypericum capitatum var, capitatum, wound dressing, ST-JOHNS-WORT, DRUG-DELIVERY SYSTEM, ALCOHOL)/CHITOSAN BLEND, ANTIMICROBIAL ACTIVITY, NANOFIBROUS SCAFFOLDS, PERFORATUM EXTRACT, FIBER DIAMETER, ESSENTIAL OILS, PROLIFERATION, DIFFERENTIATION
  • Abdullah Gül University Affiliated: Yes

Abstract

In this study, we fabricated poly(D, L-lactide-co-glycolide)/gelatin (PLGA/gelatin) membranes containing different amounts of Hypericum capitatum var. capitatum (HCC) extract (1, 5, 7.5, 10 wt%) by electrospinning technique. We investigated chemical, morphological, physical, and mechanical properties as well as in vitro degradation behavior of the electrospun membranes. We also evaluated the antibacterial activity of the electrospun membranes against Escherichia coli and Staphylococcus aureus. Viability, adhesion, and attachment of human fibroblast cells on the electrospun membranes on pre-set days were evaluated by the colorimetric CellTiter 96(R) AQueous One Solution Cell Proliferation Assay (MTS assay), scanning electron microscopy (SEM), and 4',6-Diamidino-2-Phenylindole (DAPI) staining.