Elevated IL-4 and IFN-γ levels in muscle tissue of patients with dermatomyositis

GİRİŞ M., Durmuş H., Yetimler B., Taşli H., Parman Y., TÜZÜN E.

In Vivo, vol.31, no.4, pp.657-660, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 4
  • Publication Date: 2017
  • Doi Number: 10.21873/invivo.11108
  • Journal Name: In Vivo
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.657-660
  • Keywords: Dermatomyositis, ELISA, Inerleukin-17, Interferon-gamma, Interleukin-4
  • Abdullah Gül University Affiliated: No

Abstract

Background/Aim: To investigate the contribution of muscle tissue-derived cytokines in dermatomyositis (DM). Materials and Methods: Muscle homogenates were prepared from deltoid muscle biopsy specimens of 10 patients with DM and eight controls with no pathological signs of myopathy. Interleukin (IL)-4, interferon (IFN)- γ and IL-17 levels were evaluated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting analysis. Muscle strength grades were recorded. Results: Patients with DM showed significantly elevated muscle tissue IL-4 and IFN-γ levels, whereas IL-17 levels were comparable between patients with DM and controls. Immunoblotting studies confirmed ELISA results. In DM muscle specimens, IL-4 and IFN-γ levels were positively correlated, while no correlation was observed between IL-17 and the other two cytokines. Moreover, IL-4 and IFN-γ levels were significantly negative correlated with muscle strength grades for the deltoid muscle. Conclusion: Our results confirm the involvement of T helper (Th) 1-type and Th2-type immunity in DM pathogenesis. Muscle tissue appears to contribute to muscle weakness in DM by producing inflammatory cytokines.