A photocleavable surfactant for top-down proteomics

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Brown K. A., Chen B., Guardado-Alvarez T. M., Lin Z., Hwang L., Ayaz-Guner Ş., ...Daha Fazla

NATURE METHODS, cilt.16, sa.5, ss.417-423, 2019 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 5
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1038/s41592-019-0391-1
  • Dergi Adı: NATURE METHODS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.417-423
  • Abdullah Gül Üniversitesi Adresli: Evet

Özet

We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to that of sodium dodecyl sulfate (SDS) and is compatible with mass spectrometry. Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo is simple to synthesize and can be used as a general SDS replacement in SDS-polyacrylamide gel electrophoresis.