Specific Enrichment of Phosphoproteins Using Functionalized Multivalent Nanoparticles

Creative Commons License

Hwang L., Ayaz-Guner Ş., Gregorich Z. R., Cai W., Valeja S. G., Jin S., ...Daha Fazla

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, cilt.137, sa.7, ss.2432-2435, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 137 Sayı: 7
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1021/ja511833y
  • Dergi Adı: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2432-2435
  • Abdullah Gül Üniversitesi Adresli: Hayır

Özet

Analysis of protein phosphorylation remains a significant challenge due to the low abundance of phosphoproteins and the low stoichiometry of phosphorylation, which requires effective enrichment of phosphoproteins. Here we have developed superparamagnetic nanoparticles (NPs) whose surface is functionalized by multivalent ligand molecules that specifically bind to the phosphate groups on any phosphoproteins. These NPs enrich phosphoproteins from complex cell and tissue lysates with high specificity as confirmed by SDS-PAGE analysis with a phosphoprotein-specific stain and mass spectrometry analysis of the enriched phosphoproteins. This method enables universal and effective capture, enrichment, and detection of intact phosphoproteins toward a comprehensive analysis of the phosphoproteome.