This study is conducted to characterize some nutritional attributes and starch properties of 156 Turkish sorghum populations and 4 standard cultivars (Sorghum bicolor L.). Crude protein contents of the populations vary between 6.67% and 14.33%, digestible protein ratios between 6.67% and 82.24%, crude oil contents between 2.15% and 6.40%, phytic acid contents between 0.37% and 4.09%, resistant starch between 1.10% and 34.23%, nonresistant starch between 10.79% and 79.61%, total starch between 15.42% and 85.54%, amylose between 5.67% and 43.48%, amylopectin between 9.45% and 65.67%, total phenolic between 0.19% and 5.06 mg GAE/g and antiradical activity between 3.72% and 91.48%. Significant differences are obtained from starch-based Rapid Visco Analyzer parameters of sorghum genotypes. As compared standard cultivars, several superior genotypes are identified in terms of nutritional characteristics. Genotype treatment (GT) biplot analysis revealed ideal genotypes for investigated parameters. Present findings confirmed that there are many genotypes with superior nutritional properties in local sorghum genotypes.