Chitosan scaffolds with BMP-6 loaded alginate microspheres for periodontal tissue engineering

Soran Z. , Aydin R. S. T. , GÜMÜŞDERELİOĞLU M.

JOURNAL OF MICROENCAPSULATION, cilt.29, sa.8, ss.770-780, 2012 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Konu: 8
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3109/02652048.2012.686531
  • Sayfa Sayıları: ss.770-780


The aim of this study is to develop an effective growth factor releasing scaffold-microsphere system for promoting periodontal tissue engineering. Bone morphogenetic protein-6 (BMP-6)-loaded alginate microspheres in narrow size distribution were produced by optimising electrospraying conditions. The addition of these microspheres to chitosan gels produced a novel scaffold in which not only the pore sizes and inter-connectivity were preserved, but also a controlled release vehicle was generated. Loading capacity was adjusted as 50 ng or 100 ng BMP-6 for each scaffold and the controlled release behaviour of BMP-6 from chitosan scaffolds was observed during seven days. Cell culture studies were carried out with rat mesenchymal stem cells derived from bone marrow in three groups; chitosan scaffolds, chitosan scaffolds containing BMP-6-loaded alginate microspheres and chitosan scaffolds with free BMP-6 in culture medium. Results showed that controlled delivery of BMP-6 from alginate microspheres has a significant effect on osteogenic differentiation.