Suppression of inflammatory cytokines expression with bitter melon (Momordica charantia) in TNBS-instigated ulcerative colitis
JOURNAL OF TRANSLATIONAL INTERNAL MEDICINE, cilt.8, sa.3, ss.177-187, 2020 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 8 Sayı: 3
- Basım Tarihi: 2020
- Doi Numarası: 10.2478/jtim-2020-0027
- Dergi Adı: JOURNAL OF TRANSLATIONAL INTERNAL MEDICINE
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
- Sayfa Sayıları: ss.177-187
- Anahtar Kelimeler: Momordica charantia, ulcerative colitis, inflammatory bowel disease, anti-inflammatory, inflammatory cytokines, vitamin D, CYP27B1, trinitrobenzenesulfonic acid, immunohistochemistry, alternative and complementary therapeutics, NF-KAPPA-B, INTESTINAL INFLAMMATION, SIGNALING PATHWAY, GENE-EXPRESSION, CROHNS-DISEASE, RAT MODEL, VITAMIN-D, PROTEIN, IL-23, PROTECTION
- Abdullah Gül Üniversitesi Adresli: Hayır
Özet
Background and Objective: This study was aimed to elucidate the molecular mechanism of Momordica charantia (MCh), along with a standard drug prednisolone, in a rat model of colitis induced by trinitrobenzene sulfonic acid (TNBS). Methods: After the induction of the experimental colitis, the animals were treated with MCh (4 g/kg/day) for 14 consecutive days by intragastric gavage. The colonic tissue expression levels of C-C motif chemokine ligand 17 (CCL-17), interleukin (IL)-1 beta, IL-6, IL-23, interferon-gamma (IFN-gamma), nuclear factor kappa B (NFkB), and tumor necrosis factor-alpha (TNF-alpha), were determined at both mRNA and protein levels to estimate the effect of MCh. Besides, colonic specimens were analyzed histopathologically after staining with hematoxylin and eosin. Results: The body weights from TNBS-instigated colitis rats were found to be significantly lower than untreated animals. Also, the IFN-gamma, IL-1 beta, IL-6, Il-23, TNF-alpha, CCL-17, and NF-kB mRNA and protein levels were increased significantly from 1.86-4.91-fold and 1.46-5.50-fold, respectively, in the TNBS-instigated colitis group as compared to the control. Both the MCh and prednisolone treatment significantly reduced the bodyweight loss. It also restored the induced colonic tissue levels of IL-1 beta, IL-6, IFN-gamma, and TNF-alpha to normal levels seen in untreated animals. These results were also supported with the histochemical staining of the colonic tissues from both control and treated animals. Conclusion: The presented data strongly suggests that MCh has the anti-inflammatory effect that might be modulated through vitamin D metabolism. It is the right candidate for the treatment of UC as an alternative and complementary therapeutics.