Gene delivery: intelligent but just at the beginning


PISKIN E., Dincer S. , TURK M.

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, vol.15, no.9, pp.1181-1202, 2004 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 15 Issue: 9
  • Publication Date: 2004
  • Doi Number: 10.1163/1568562041753016
  • Title of Journal : JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
  • Page Numbers: pp.1181-1202

Abstract

Gene therapy is used to treat genetic disorders, which may be achieved both ex vivo and in vivo. Gene-delivery systems usually include a carrier system which both protects the gene expression plasmid and allows its extracellular and intracellular trafficking. Viruses are used in most of the clinical trials today; however, they do have important drawbacks. Non-viral vectors based on lipids, water-soluble polycations, other non-condensing polymers and nano- or microparticles/capsules have been proposed. Cationic polymers. especially carrying novel targeting ligands. are receiving increasing attention. Intelligent polymers with temperature, pH, and light sensitivities for a controllable and effective non-viral transfection have recently been introduced but are just at the beginning. Our preliminary studies showed that block copolymers of N-isopropylacrylamide-acrylic acid with poly(ethylene imine) could be one example of these novel non-viral vectors.