Research Information System
in: Applications of Plant Molecular Farming, Chittaranjan Kole,Anurag Chaurasia,Kathleen L. Hefferon,Jogeswar Panigrahi, Editor, Springer, London/Berlin , Singapore, pp.235-258, 2024
The current recombinant biopharmaceutical industry has a multibillion dollar market size and is estimated to keep increasing in the near future. Recombinant pharmaceutical proteins are produced in heterologous host organisms, including bacteria, fungi, plant, insects, and mammalian cells. Although the current production systems for the recombinant biopharmaceuticals mostly rely on the mammalian cells, transgenic plants exhibit alternative platforms with low-cost and high-yield production. Scaling up the production of recombinant proteins in plants for large-scale manufacturing is also readily achievable. This chapter summarizes some of the factors influencing the expression of recombinant biopharmaceuticals in various host organisms. Particular emphasis was placed on highlighting the advantages of using plants as host organisms, strategies for expressing recombinant proteins, and optimizing protein expression in plant systems. The successful examples for the recombinant production of pharmaceutical proteins in plant chloroplasts were also summarized.