A Laser-Assisted Cellular Electrophysiology Measurement System

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Seymen A. A., Ozgur E., Soran-Erdem Z., Ortac B.

IEEE PHOTONICS TECHNOLOGY LETTERS, cilt.33, sa.3, ss.163-166, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 3
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1109/lpt.2021.3049348
  • Dergi Adı: IEEE PHOTONICS TECHNOLOGY LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.163-166
  • Abdullah Gül Üniversitesi Adresli: Hayır

Özet

Patch-clamp technique is the gold standard for cellular electrophysiological measurements, which is capable of measuring single ion transport events across the cell membrane. However, the measurement possesses significant complexities, and it requires a high level of expertise, while its experimental throughput is nevertheless considerably low. Here, we suggest and experimentally demonstrate a laser-assisted method for performing cellular electrophysiological measurements. Femtosecond laser pulses, coupled to an optical microscope, are used to form a sub-micrometer hole on a thin polymer membrane separating two electrodes, where a nearby cell is subsequently placed onto the hole by negative pressure. Afterwards, the cell is punctured using subsequent laser exposure, revealing the cell membrane over the hole for electrophysiological recording. This system could be used to increase the output amount of the electrophysiological measurements substantially.