Image Processing and Cell Phone Microscopy to Analyze the Immunomagnetic Beads on Micro-Contact Printed Gratings


İÇÖZ K.

APPLIED SCIENCES-BASEL, cilt.6, 2016 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 6 Konu: 10
  • Basım Tarihi: 2016
  • Doi Numarası: 10.3390/app6100279
  • Dergi Adı: APPLIED SCIENCES-BASEL

Özet

In this paper we report an ultra-low-cost spherical ball lens based cell phone microscopy and image processing algorithms to analyze the amount of immunomagnetic beads on micro-contact printed gratings. The spherical ball lens provides approximately 100x magnification but the recorded images are not clear and are noisy. By using the image-processing algorithms, the noise can be reduced and the images can be enhanced to quantify the amount of immunomagnetic beads on micro-contact printed lines. This method, which is portable and low-cost, can be an alternative read out mechanism for biosensing applications using immunomagnetic beads on micro-contact printed surface receptors. Further, 0.0335 mg/mL was the lowest magnetic bead concentration that could be detected above the inherent noise level of the spherical ball lens.