Image Processing and Cell Phone Microscopy to Analyze the Immunomagnetic Beads on Micro-Contact Printed Gratings
APPLIED SCIENCES-BASEL, cilt.6, sa.10, 2016 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 6 Sayı: 10
- Basım Tarihi: 2016
- Doi Numarası: 10.3390/app6100279
- Dergi Adı: APPLIED SCIENCES-BASEL
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Anahtar Kelimeler: cell phone microscopy, micro-contact printing, immunomagnetic beads, image processing, spherical ball lens, MICROCONTACT, PROTEINS
- Abdullah Gül Üniversitesi Adresli: Evet
Özet
In this paper we report an ultra-low-cost spherical ball lens based cell phone microscopy and image processing algorithms to analyze the amount of immunomagnetic beads on micro-contact printed gratings. The spherical ball lens provides approximately 100x magnification but the recorded images are not clear and are noisy. By using the image-processing algorithms, the noise can be reduced and the images can be enhanced to quantify the amount of immunomagnetic beads on micro-contact printed lines. This method, which is portable and low-cost, can be an alternative read out mechanism for biosensing applications using immunomagnetic beads on micro-contact printed surface receptors. Further, 0.0335 mg/mL was the lowest magnetic bead concentration that could be detected above the inherent noise level of the spherical ball lens.