Potential c-myc Antisense Oligonucleotide Carriers: PCl/PEG/PEI and PLL/PEG/PEI


Dincer S. , Turk M., Karagoz A., Uzunalan G.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, cilt.39, ss.143-154, 2011 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 39 Konu: 3
  • Basım Tarihi: 2011
  • Doi Numarası: 10.3109/10731199.2010.506852
  • Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Sayfa Sayısı: ss.143-154

Özet

In this work, positively charged, micelle-forming polymers were synthesized and used as a model vector to deliver antisense oligodeoxynucleotide (ASODN) into melanoma cells. Polymers and polymer/ASODN complexes were characterized by DLS according to size, charge, and critical micelle concentration. Nanosize and spherical complexes were observed by AFM. Complexes did not reveal significant toxicity to melanoma cells. Antiproliferative effect of the complexes was observed by immunocytochemical staining and estimated as 56.8% with N/P:9. High amount of apoptosis and very small amount of necrosis were estimated. According to the results, these positively charged polymers forming micelle-like structures seem promising as ASODN carriers.